MicroTime 200 STED Add-On
Brand: PicoQuant | Category: Nanotechnology
This open STED add-on extends the MicroTime 200 to super-resolution imaging with time-resolved contrast, resolving nanoscale structures below 50 nm that confocal images cannot separate. A stable donut shaped depletion beam is produced by an EASYDOnut phase plate, which avoids complex alignment, and gSTED time gating isolates late photons for better resolution and sensitivity. Triple-species STED imaging is possible using fluorescence pattern matching for accurate multicolour co-localisation, and the reduced observation volume benefits STED-FCS by allowing diffusion measurements at higher concentrations. Full compatibility with the MicroTime 200 is retained, and the complete STED workflow including time gating and multi-species pattern matching runs in SymPhoTime 64.
Manufacturer-listed upgrade configuration
| Parameter | Value |
|---|---|
| Host platform | MicroTime 200 |
| Method | STED super-resolution with time-gated STED capability |
| Optical element | EASYDOnut depletion-beam phase plate |
| Analysis environment | SymPhoTime 64 |
| Additional workflows | STED-FCS and multi-species pattern matching |
PicoQuant exact add-on page reviewed 29 September 2026. Resolution depends on fluorophore, sample, optics and acquisition conditions; request application-specific evidence and the compatible laser configuration. Checked 2026-09-29.
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